Volume 18, Issue 1 (Pajouhan Scientific Journal, Autumn 2019)                   psj 2019, 18(1): 15-22 | Back to browse issues page

DOI: 10.29252/psj.18.1.15

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1- Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran
2- Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran , s_ghorbian@iau-ahar.ac.ir
Abstract:   (3543 Views)
Background and Objective: The CGA gene encodes alpha subunit of glycoprotein hormones that are involved in the fertility process. The aim of this study was to evaluate the association between glycoprotein hormones, alpha polypeptide (CGA) rs6631A> T gene polymorphism with the risk of men infertility with azoospermia or severe oligozoospermia.
Materials and Methods: This study was conducted in a case-control study on 200 blood samples consisting of 100 samples of severe oligozoospermia or azoospermia men and 100 blood samples of healthy men with normal fertility and lack of a family history of infertility. In this study, we used PCR-RFLP method to evaluate genotype frequencies of CGA polymorphism.
Results: The genotype frequencies of CGA gene polymorphism for AA, TA and TT was 27%, 51% and 22% and 24%, 47% and 27%, in control and case groups, respectively. There was no significant difference in genotype frequencies between two groups (P> 0.05). In addition, there was no significant difference in allele frequency between two groups (P=0.424, OR=0.852, CI=0.576-1.261).
Conclusion: The results of this investigation showed that nucleotide change in CGA gene was not significantly associated with the risk of azoospermia or severe oligozoospermia; therefore, this polymorphism may not be a prognostic biomarker in male infertility.
Full-Text [PDF 841 kb]   (113 Downloads)    
Type of Study: Research Article | Subject: Basic Sciences
Received: 2019/08/2 | Accepted: 2019/10/9 | Published: 2019/10/2

1. Iammarrone E, Balet R, Lower AM, Gillott C, Grudzinskas JG. Male infertility. Best practice & research Clinical obstetrics & gynaecology. 2003;17(2):211-29. [DOI]
2. Anderson JE, Farr SL, Jamieson DJ, Warner L, Macaluso M. Infertility services reported by men in the United States: national survey data. Fertility and sterility. 2009;91(6):2466-70. [DOI]
3. Ahmed A, Bello A, Mbibu NH, Maitama HY, Kalayi GD. Epidemiological and aetiological factors of male infertility in northern Nigeria. Nigerian journal of clinical practice. 2010;13(2):205-9. [DOI]
4. Carpi A, Sabanegh E, Mechanick J. Controversies in the management of nonobstructive azoospermia. Fertility and sterility. 2009;91(4):963-70. [DOI]
5. Cheng YS, Lu CW, Lin TY, Lin PY, Lin YM. Causes and Clinical Features of Infertile Men With Nonobstructive Azoospermia and Histopathologic Diagnosis of Hypospermatogenesis. Urology. 2017;105:62-8. [DOI]
6. Bahadur G, Homburg R, Muneer A, Racich P, Alangaden T, Al-Habib A, et al. First line fertility treatment strategies regarding IUI and IVF require clinical evidence. Human reproduction (Oxford, England). 2016;31(6):1141-6. [DOI]
7. Abdel Raheem A, Garaffa G, Rushwan N, De Luca F, Zacharakis E, Abdel Raheem T, et al. Testicular histopathology as a predictor of a positive sperm retrieval in men with non-obstructive azoospermia. BJU International. 2013;111(3):492-9. [DOI]
8. Bieche I, Latil A, Parfait B, Vidaud D, Laurendeau I, Lidereau R, et al. CGA gene (coding for the alpha subunit of glycoprotein hormones) overexpression in ER alpha-positive prostate tumors. Eur Urol. 2002;41(3):335-41. [DOI]
9. Kendall SK, Samuelson LC, Saunders TL, Wood RI, Camper SA. Targeted disruption of the pituitary glycoprotein hormone alpha-subunit produces hypogonadal and hypothyroid mice. Genes & development. 1995;9(16):2007-19. [DOI]
10. Zhang H, Liu Y, Su D, Yang Y, Bai G, Tao D, et al. A single nucleotide polymorphism in a miR-1302 binding site in CGA increases the risk of idiopathic male infertility. Fertility and sterility. 2011;96(1):34-9.e7. [DOI]
11. Troppmann B, Kossack N, Nordhoff V, Schuring AN, Gromoll J. MicroRNA miR-513a-3p acts as a co-regulator of luteinizing hormone/chorionic gonadotropin receptor gene expression in human granulosa cells. Mol Cell Endocrinol. 2014;390(1-2):65-72. [DOI]
12. Bernstein LR, Mackenzie AC, Lee SJ, Chaffin CL, Merchenthaler I. Activin Decoy Receptor ActRIIB:Fc Lowers FSH and Therapeutically Restores Oocyte Yield, Prevents Oocyte Chromosome Misalignments and Spindle Aberrations, and Increases Fertility in Midlife Female SAMP8 Mice. Endocrinology. 2016;157(3):1234-47. [DOI]
13. Ghorbian S. Micro-RNAs, next-generation molecular markers in male infertility field. Translational andrology and urology. 2012;1(4):245-6. [DOI]
14. Kian R, Moradi S, Ghorbian S. Role of components of microRNA machinery in carcinogenesis. Experimental oncology. 2018;40(1):2-9. [DOI]
15. Ogorevc J, Dovc P, Kunej T. Polymorphisms in microRNA targets: a source of new molecular markers for male reproduction. Asian journal of andrology. 2011;13(3):505-8. [DOI]
16. Khazaie Y, Esfahani MHN. MicroRNA and male infertility: a potential for diagnosis. International journal of fertility & sterility. 2014;8(2):113. [DOI]
17. Mohseni Sani N, Sheidai M, Noormohammadi Z, Kalhor N, Atri Roozbahani G. Study of association the single nucleotide polymorphisms (rs6631) in 3′-UTR CGA gene with male infertility in Iranian population. Gene Reports. 2018;12:61-5. [DOI]
18. Jamalvandi M, Motovali-Bashi M, Amirmahani F, Darvishi P, Jamshidi Goharrizi K. Association of T/A polymorphism in miR-1302 binding site in CGA gene with male infertility in Isfahan population. Molecular biology reports. 2018;45(4):413-7. [DOI]
19. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988;16(3):1215. [DOI]
20. Miyamoto T, Minase G, Shin T, Ueda H, Okada H, Sengoku K. Human male infertility and its genetic causes. Reprod Med Biol. 2017;16(2):81-8. [DOI]
21. Miyamoto T, Minase G, Okabe K, Ueda H, Sengoku K. Male infertility and its genetic causes. J Obstet Gynaecol Res. 2015;41(10):1501-5. [DOI]
22. Poursadegh A, Poursadegh AA, Ghorbian S. PiRNAs interacting proteins, candidate molecular marker for evaluation of idiopathic male infertility. Andrologia. 2014: 1;46(8):823. [DOI]