RT - Journal Article T1 - The evaluation of bacterial contamination of ophtalmic biomicroscopy apparatus (Slit-lamp) in eye centers of Farshcian Hospital in Hamadan and assessment of the current disinfectants JF - umsha-psj YR - 2014 JO - umsha-psj VO - 12 IS - 2 UR - http://psj.umsha.ac.ir/article-1-35-en.html SP - 18 EP - 27 K1 - Disinfectants K1 - Bacterial contamination K1 - Slit-lamp K1 - Sodium hypochlorite K1 - Chlorohexidine AB - Background and Objectives: The aim of this study is the evaluation of bacterial contamination of Slit-lamps in Eye Centers of Farshchian Hospital and assessment of the current disinfectants on isolated bacteria from apparatuses. Methods: In a cross-sectional study, 400 samples were collected from different parts of Slit-lamps (200 samples before and 200 samples after disinfection). The samples were cultured on Blood agar by sterile cotton swabs. A smear was also prepared for gram staining, then by differential and biochemical test identify and diagnosis microbial agents. The efficacy of two disinfectants (Sodium hypochlorite and Chlorohexidine) on Staphilococus epidermidis and Corynebacterium diphteroides by disk diffusion method were evaluated. Data was gathered through a questionnaire and analyzed by SPSS software (V:10) and T-test. Results: The rate of slit-lamp's microbial contamination before and after disinfection was 87% and 42% respectively, that showed significant decrease after disinfection (p LA eng UL http://psj.umsha.ac.ir/article-1-35-en.html M3 ER -