Introduction: Nowadays, workforce, as the most valuable asset, is facing many problems. Stressors in workforce, which have adverse effects on the body and spirit of nurses, can result in the performance of staff. The objective of this study was to determine stressors in operating room and its impacts on nurses.

Methods: This research was a cross-sectional study and the study population consisted of 172 operating room nurses working in five hospitals of Hamadan University of Medical sciences. The individuals were selected by using the total number of sampling method. The instrument for data collecting was a questionnaire that has checked stressors in five domains: psycho - social, economic and welfare, environmental, managerial and physical factors.For data analysis, SPSS 16 software, descriptive statistics, and independent t tests and correlation were used.

Results: Results showed that 62.2% of the individuals were exposed to high stress. Psychological and environmental factors had the highest levels of stress (59.21, 39.65) and the managerial domain had the lowest level of stress (17.7). Among operating room stressors, the risk of injuries from contaminated needles and the damage to the skin (50%) was most likely to cause stress.

Conclusion: The present study showed that more than half of the nurses suffer from high levels of stress and serious actions should be taken in relation to the manage mental and environmental domains. It is suggested that in order to support operating room nurses against occupational stressors, some interventions should be designed and performed.