Introduction: Herpes simplex virus infection is one of the most common human infections in most parts of the world. Infection of female genitalia, as a common involving region in body, may cause neonatal infection to vitus and lead to dangerous complications. The aim of the current research was the assessment of the seroepidemiology of herpes simplex virus (HSV) in females referring to genetic center of Shahid Beheshti hospital in Hamadan from 2005 to 2010.

Methods: In this retrospective descriptive study, from total of 5700 females, referring to the genetic center of ShahidBeheshti Hospital in Hamadan between 2005-2009, 921 females who their medical data of HSV tests were available in their records were studied. The collected data were analyzed using Chi square test, univariate and multivariate logistic regression models and SPSS version 16 software.

Results: The results showed that 5.3% of the females referring to genetic center of Shahid Beheshti Hospital of hamadn were seropositive for hsv-2 antibody. The trend for change in prevalence of seropostivity of HSV 2 was significant and there was a meaningful relationship between marriage status and positivity of Hsv2 antibody among females.

Conclusion: It seems that the prevalence of HSV-2 infection has had a decreased trend in recent years. The prevalence rate of HSV-2 was in consistent with other parts of the country, which may be due to the awareness of people of transmission routs, prevention as well as treatment methods.