Volume 18, Issue 2 (Pajouhan Scientific Journal, Winter 2020)                   psj 2020, 18(2): 81-89 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zarei M, Izadi Dastenaei Z, Jabbari S. Pain Relief and Kaempferol: Activation of Transient Receptors Potential Vanilloid Type 1 in Male Rats. psj. 2020; 18 (2) :81-89
URL: http://psj.umsha.ac.ir/article-1-562-en.html
1- Associate Professor of Physiology, Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , zarei@umsha.ac.ir
2- MSc of Physiology, Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
3- PhD Student of Animal Physiology, Department of Biology, Faculty of Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
Abstract:   (778 Views)
Background and Objective: Pain an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage. Kaempferol is one of the most important herbal active constituent with antinociceptive effects. The aim of this study was to evaluate the effects of intracerebroventricular injection of kaempferol and its interaction with the transient receptor potential cation channel subfamily V member 1 (TRPV1) on pain in male rats.
Materials and Methods: In this experimental study, sixty male rats (200-250 g) were divided to the following groups: control (saline), Dimethyl sulfoxide (DMSO), morphine, kaempferol at dosages of 0.5, 1, and 1.5 mg/rat, capsaicin, capsazepine, capsaicin plus kaempferol (1.5 mg/rat), capsazepine plus kaempferol (1.5 mg/rat). After cannula implantation in cerebroventricular area, the rats received target components and then evaluated by pain assessment tests (abdominal writhing, tail-flick, and formalin tests). Data were analyzed by one-way ANOVA followed by Tukey’s post-test and P<0.05 was as a significant difference.
Results: The results showed that administration of kaempferol  had significant analgesic effects in comparison to the control/DMSO groups on the tail-flick, abdominal writhing, and formalin tests (P<0.05). Co-administration of capsaicin and kaempferol (1.5 mg/ rat) had significant analgesic effects compared to the control/DMSO groups although, not a synergist. Moreover, co-administration of capsazepine and kaempferol (1.5 mg/ rat) mostly decreased antinociceptive effects of kaempferol.
Conclusion: The kaempferol probably has both acute and inflammatory antinociceptive effects and exert this activity at least in part by activating TRPV1 receptors.
Full-Text [PDF 790 kb]   (51 Downloads)    
Type of Study: Research Article | Subject: Basic Sciences
Received: 2020/01/7 | Accepted: 2020/02/6 | Published: 2020/01/10

1. 1. Huang T, Lin S-H, Malewicz NM, Zhang Y, Zhang Y, Goulding M, et al. Identifying the pathways required for coping behaviours associated with sustained pain. Nature. 2019;565(7737):86. [DOI]
2. 2. Liu N-J, Storman EM, Gintzler AR. Pharmacological modulation of endogenous opioid activity to attenuate neuropathic pain in rats. The Journal of Pain. 2019;20(2):235-43. [DOI]
3. 3. Zarei M, Mohammadi S, Shahidi S, Fallahzadeh AR. Effects of Sonchus asper and apigenin-7-glucoside on nociceptive behaviors in mice. Journal of Pharmacy & Pharmacognosy Research. 2017;5(4):227-37. [DOI]
4. 4. Golshani Y, Zarei M, Mohammadi S. Acute/chronic pain relief: Is Althaea officinalis essential oil effective. Avicenna Journal of Neuro Psych Physiology. 2015;2(4). [DOI]
5. 5. Fallahzadeh A, Mohammadi S. An investigation of the antinociceptive and anti-inflammatory effects of hydroalcoholic extract of Inula helenium on male rats. Journal of Babol University of Medical Sciences. 2016;18(12):57-63. [DOI]
6. 6. Imran M, Rauf A, Shah ZA, Saeed F, Imran A, Arshad MU, et al. Chemo‐preventive and therapeutic effect of the dietary flavonoid kaempferol: A comprehensive review. Phytotherapy research. 2019;33(2):263-75. [DOI]
7. 7. Abo-Salem OM. Kaempferol attenuates the development of diabetic neuropathic pain in mice: Possible anti-inflammatory and anti-oxidant mecha-nisms. Macedonian Journal of Medical Sciences. 2014;7(3):424-30. [DOI]
8. 8. Kishore L, Kaur N, Singh R. Effect of Kaempferol isolated from seeds of Eruca sativa on changes of [DOI]
9. pain sensitivity in Streptozotocin-induced diabetic neuropathy. Inflammopharmacology. 2018;26(4):993-1003. [DOI]
10. 9. Goldberg DS, McGee SJ. Pain as a global public health priority. BMC public health. 2011;11(1):1. [DOI]
11. 10. Mohammadi S, Golshani Y. Neuroprotective Effects of Rhamnazin as a Flavonoid on Chronic Stress-Induced Cognitive Impairment. Journal of Advanced Neuroscience Research. 2017;4:30-7. [DOI]
12. 11. Wang x, Yu z, He z, Zhang q, Yu s. Intracerebroventricular infusion of D-serine decreases nociceptive behaviors induced by electrical stimulation of the dura mater of rat. Neurological research. 2019;41(3):204-7. [DOI]
13. 12. Christoph T, Grünweller A, Mika J, Schäfer MK-H, Wade EJ, Weihe E, et al. Silencing of vanilloid receptor TRPV1 by RNAi reduces neuropathic and visceral pain in vivo. Biochemical and biophysical research communications. 2006;350(1):238-43. [DOI]
14. 13. Jara-Oseguera A, Simon SA, Rosenbaum T. TRPV1: on the road to pain relief. Current molecular pharmacology. 2008;1(3):255-69. [DOI]
15. 14. Mahmoodi M, Mohammadi S, Zarei M. Antinociceptive effect of hydroalcoholic leaf extract of tribulus terrestris L. in male rat. Journal of Babol University of Medical Sciences. 2013;15(6):36-43. [DOI]
16. 15. Zhou Q, Bao Y, Zhang X, Zeng L, Wang L, Wang J, et al. Optimal interval for hot water immersion tail-flick test in rats. Acta neuropsychiatrica. 2014; [DOI]
17. 26(4):218-22. [DOI]
18. 16. McGaraughty S, Chu KL, Bitner RS, Martino B, Kouhen RE, Han P, et al. Capsaicin infused into the PAG affects rat tail flick responses to noxious heat and alters neuronal firing in the RVM. Journal of neurophysiology. 2003;90(4):2702-10. [DOI]
19. 17. Asgari Neamatian M, Yaghmaei P, Mohammadi S. Assessment of the antinociceptive, antiinflammatory and acute toxicity effects of Ducrosia anethifolia essential oil in mice. Scientific Journal of Kurdistan University of Medical Sciences. 2017; [DOI]
20. 22(3):74-84. [DOI]
21. 18. García-Mediavilla V, Crespo I, Collado PS, Esteller A, Sánchez-Campos S, Tuñón MJ, et al. The anti-inflammatory flavones quercetin and kaempferol cause inhibition of inducible nitric oxide synthase, cyclooxygenase-2 and reactive C-protein, and down-regulation of the nuclear factor kappaB pathway [DOI]
22. in Chang Liver cells. European journal of pharmacology. 2007;557(2-3):221-9. [DOI]
23. 19. Cobzaru A. High-concentration capsaicin patch (qutenza)-a new step in treatment of neuropathic pain. Amaltea Medical, Editura Magister; 2012. [DOI]
24. 20. Zarei M, Mohammadi S, Komaki A. Antinociceptive activity of Inula britannica L. and patuletin: In vivo and possible mechanisms studies. Journal of ethnopharmacology. 2018;219:351-8. [DOI]
25. 21. Komatsu T, Katsuyama S, Takano F, Okamura T, Sakurada C, Tsuzuki M, et al. Possible involvement of the μ opioid receptor in the antinociception induced by sinomenine on formalin-induced nociceptive behavior in mice. Neuroscience letters. 2019;699:103-8. [DOI]
26. 22. Zygmunt PM, Ermund A, Movahed P, Andersson DA, Simonsen C, Jönsson BA, et al. Monoacylgly-cerols activate TRPV1–a link between phospholipase C and TRPV1. PLoS One. 2013;8(12):e81618. [DOI]
27. 23. Zygmunt PM, Ermund A, Movahed P, Andersson DA, Simonsen C, Jönsson BA, et al. Monoacylgly-cerols activate TRPV1–a link between phospholipase C and TRPV1. 2013;8(12):e81618. [DOI]
28. 24. Mallet C, Barrière DA, Ermund A, Jönsson BA, Eschalier A, Zygmunt PM, et al. TRPV1 in brain is involved in acetaminophen-induced antinociception. PLoS one. 2010;5(9):e12748. [DOI]
29. 25. Guindon J, Blanton H, Rodriguez-Garcia D, Lugo I, Lilley J, Brelsfoard J. (282) Sex Differences in [DOI]
30. the Development of Tolerance to Arachidonyl-2-Chloroethalamide (ACEA) in the Mouse Formalin Pain Model. The Journal of Pain. 2019;20(4):S45. [DOI]
31. 26. Pingle S, Matta J, Ahern G. Capsaicin receptor: TRPV1 a promiscuous TRP channel. Transient Receptor Potential (TRP) Channels: Springer; 2007. p. 155-71. [DOI]
32. 27. Liao HT, Lee HJ, Ho YC, Chiou LC. Capsaicin in [DOI]
33. the periaqueductal gray induces analgesia via metabotropic glutamate receptor‐mediated endocan-nabinoid retrograde disinhibition. British journal of pharmacology. 2011;163(2):330-45. [DOI]
34. 28. Marzo VD, Starowicz K, Cristino L. TRPV1 receptors in the central nervous system: potential for previously unforeseen therapeutic applications. Current pharmaceutical design. 2008;14(1):42-54. [DOI]
35. 29. Xu N, Wang H, Fan L, Chen Q. Supraspinal administration of apelin-13 induces antinociception via the opioid receptor in mice. Peptides. 2009; [DOI]
36. 30(6):1153-7. [DOI]
37. 30. Lv S-Y, Yang YJ, Hong S, Wang N-B, Qin Y-J, Li W-X, et al. Intrathecal apelin-13 produced different actions in formalin test and tail-flick test in mice. Protein and peptide letters. 2013;20(8):926-31. [DOI]
38. 31. Lv S-Y, Qin Y-J, Wang N-B, Yang Y-J, Chen QJP. Supraspinal antinociceptive effect of apelin-13 in a mouse visceral pain model. 2012;37(1):165-70. [DOI]
39. 32. Xu N, Wang H, Fan L, Chen QJP. Supraspinal administration of apelin-13 induces antinociception via the opioid receptor in mice. 2009;30(6):1153-7. [DOI]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | Pajouhan Scientific Journal

Designed & Developed by : Yektaweb